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I've been somewhat curious about this..but.
Spoilers:
In Borderlands 2, Roland dies; shot and killed by Handsome Jack.
In this case, why couldn't he use the New-U respawn technology? In Borderlands 1, he was able to use it (in fact, all 4 of the original characters could do so), but in Borderlands 2, it seems like death is permanent, instead of being temporary.
What explains this?